Nucleic Acid Extraction Kit (A02)
Kev tshawb nrhiav txoj cai
Tom qab tso cov genomic DNA los ntawm kev faib cov hlwb nrog cov lysis tsis, cov hlaws hlau nplaum tuaj yeem xaiv khi rau genomic DNA hauv cov qauv.Ib qho me me ntawm impurities uas tau nqus los ntawm cov hlaws hlau nplaum tuaj yeem raug tshem tawm los ntawm kev ntxuav tsis huv.Hauv TE, cov hlaws sib nqus tuaj yeem tso cov boundgenome DNA, tau txais cov genome DNA zoo.Txoj kev no yog qhov yooj yim thiab ceev thiab cov txiaj ntsig DNA tau zoo yog siab, uas tuaj yeem ua tau raws li qhov yuav tsum tau muaj rau kev tshawb nrhiav DNA methylation.Lub caij no, cov khoom siv rho tawm raws li cov hlaws sib nqus tuaj yeem sib xws nrog kev rho tawm cov nucleic acid tsis siv neeg, ua tau raws li kev ua haujlwm siab ntawm nucleic acid extraction.
Cov khoom tseem ceeb ntawm cov reagent
Cov khoom yog qhia hauv kab lus 1:
Table 1 Reagent Cheebtsam thiab Chaw thau khoom
| Cov khoom npe | Cov khoom tseem ceeb | Loj (48) | Loj (200) |
| 1. Digestion Buffer A | Tris, SDS | 15.8 mL / lub raj mis | 66ml / lub raj mis |
| 2. Lysis Buffer L | Guanidinium Isothiocyanate, Tris | 15.8 mL / lub raj mis | 66ml / lub raj mis |
| 3. Ntxuav Buffer A | NaCl, Tris | 11 mL / lub raj mis | 44 mL / lub raj mis |
| 4. Ntxuav Buffer B | NaCl, Tris | 13 mL / lub raj mis | 26.5ml / lub raj mis * 2 |
| 5. TSI | Tris, EDTA | 12 mL / lub raj mis | 44 mL / lub raj mis |
| 6. Protease K daws | Protease K | 1.1 mL / daim | 4.4ml / pcs |
| 7. Sib nqus hlaws ncua kev kawm 2 | Sib nqus hlaws | 0.5 ml / pcs | 2.2 mL / daim |
| 8. Cov lus qhia rau kev rho tawm nucleic acid reagents | / | 1 luam | 1 luam |
Cheebtsam uas yuav tsum tau nyob rau hauv lub nucleic acid extraction, tab sis tsis muaj nyob rau hauv cov khoom siv:
1. Reagent: Anhydrous ethanol, isopropanol, thiab PBS;
2. Consumables: 50mL centrifuge raj thiab 1.5mL EP raj;
3. Cov khoom siv: da dej da dej, pipettes, magnetic txee, centrifuge, 96-zoo phaj (tsis siv neeg), tsis siv neeg nucleic acid extraction khoom (automatic).
Cov ntaub ntawv yooj yim
Cov qauv xav tau:
1.Qhov kev kuaj pom yuav tsum ua kom tiav nyob rau hauv 7-hnub cia ntawm ambient kub tom qab kev sau ntawm lub ncauj tsev menyuam exfoliated cell qauv (tsis-taw).
2.Qhov kev kuaj pom yuav tsum ua kom tiav nyob rau hauv 30-hnub cia ntawm ambient kub tom qab sau ntawm lub ncauj tsev menyuam exfoliated cell qauv (tso)..
3.Qhov kev kuaj pom yuav tsum ua kom tiav nyob rau hauv 30-hnub cia ntawm ambient kub tom qab sau cov zis kuaj;Kev kuaj xyuas yuav tsum ua kom tiav nyob rau lub sijhawm tom qab kev sau cov qauv ntawm cov kab ke.
Chaw nres tsheb specification:200 pcs / box, 48 pcs / thawv.
Cia tej yam kev mob:2-30 ℃
Lub sijhawm siv tau:12 hli
Khoom siv tau:Tianlong NP968-C nucleic acid extraction instrument, Tiangen TGuide S96 nucleic acid extraction instrument, GENE DIAN EB-1000 nucleic acid extraction instrument.
Cov ntaub ntawv kho mob daim ntawv pov thawj No./product technical requirements No .:HJXB No. 20210100.
Hnub pom zoo thiab hloov kho cov lus qhia:Hnub pom zoo: Kaum Ib Hlis 18, 2021
Txog Peb
Raws li lub tuam txhab high-tech nrhiav tau nyob rau hauv 2018 los ntawm cov kws tshaj lij epigenetic sab saum toj, Epiprobe tsom rau kev kuaj mob molecular ntawm cancer DNA methylation thiab precision theranostics kev lag luam.Nrog rau kev siv thev naus laus zis zoo, peb lub hom phiaj los coj lub sijhawm ntawm cov khoom siv tshiab rau kev mob qog noj ntshav hauv lub paj!
Raws li Epiprobe core team txoj kev tshawb fawb mus sij hawm ntev, kev txhim kho thiab kev hloov pauv hauv thaj chaw ntawm DNA methylation nrog cov kev hloov kho tshiab, ua ke nrog cov cim DNA methylation lub hom phiaj ntawm cov qog nqaij hlav, peb siv cov cim sib txawv ntau yam sib xyaw ua ke nrog cov ntaub ntawv loj thiab kev txawj ntse thev naus laus zis rau nws tus kheej tsim ib qho tshwj xeeb patent-tiv thaiv kua biopsy technology.Los ntawm kev txheeb xyuas cov qib methylation ntawm cov chaw tshwj xeeb ntawm cov kab mob DNA dawb hauv cov qauv, cov kev tsis txaus ntawm cov kev kuaj mob ib txwm muaj thiab cov kev txwv ntawm kev phais thiab kev ntsuas qhov ntsuas tau raug zam, uas tsis yog tsuas yog ua tiav qhov tseeb ntawm kev kuaj mob qog noj ntshav thaum ntxov, tab sis kuj tseem tuaj yeem saib xyuas lub sijhawm. ntawm kev mob qog noj ntshav thiab kev loj hlob dynamics.
