TAGMe DNA Methylation Detection Kits (qPCR) rau Cervical Cancer / Endometrial Cancer
KHOOM NTA
Tsis-invasive
Muaj feem xyuam nrog lub ncauj tsev menyuam txhuam thiab Pap smear kuaj.
Yooj yim
Thawj Me-qPCR methylation nrhiav tshuab tuaj yeem ua tiav hauv ib kauj ruam hauv 3 teev yam tsis muaj kev hloov pauv bisulfite.
Thaum ntxov
Detectable nyob rau theem precancerous.
Automation
Nrog rau cov txiaj ntsig kev tshuaj ntsuam xyuas software, kev txhais cov txiaj ntsig yog automated thiab nyeem tau ncaj qha.
Daim ntawv thov scenarios
Kev tshuaj ntsuam thaum ntxov
Cov neeg noj qab haus huv
Kev soj ntsuam kev pheej hmoo mob qog noj ntshav
Cov neeg muaj kev pheej hmoo siab (zoo rau kev pheej hmoo siab human papillomavirus (hrHPV) lossis zoo rau lub ncauj tsev menyuam exfoliation cytology / zoo rau kev pheej hmoo siab human papillomavirus (hrHPV) lossis zoo rau lub ncauj tsev menyuam exfoliation cytology)
Kev soj ntsuam rov tshwm sim
Prognostic pejxeem
YUAV TSUM SIV
Cov khoom siv no yog siv rau hauv vitro qualitative nrhiav pom ntawm hypermethylation ntawm cov noob PCDHGB7 nyob rau hauv cov qauv ncauj tsev menyuam.Rau kev mob qog noj ntshav hauv tsev menyuam, qhov txiaj ntsig zoo qhia tau tias muaj kev pheej hmoo siab ntawm qib 2 lossis qib siab dua / ntau dua ntawm ncauj tsev menyuam intraepithelial neoplasia (CIN2+, suav nrog CIN2, CIN3, adenocarcinoma hauv situ, thiab mob qog noj ntshav), uas yuav tsum tau kuaj ntxiv colposcopy thiab / lossis histopathological kuaj. .Ntawm qhov tsis sib xws, cov txiaj ntsig tsis zoo qhia tau hais tias qhov kev pheej hmoo ntawm CIN2+ tsawg, tab sis qhov kev pheej hmoo tsis tuaj yeem raug cais tawm tag nrho.Kev kuaj mob zaum kawg yuav tsum yog nyob ntawm colposcopy thiab / lossis cov txiaj ntsig histopathological.Ntxiv mus, rau kev mob qog noj ntshav endometrial, qhov txiaj ntsig zoo qhia tau tias muaj kev pheej hmoo ntawm endometrial precancerous lesions thiab mob qog noj ntshav, uas yuav tsum tau kuaj histopathological ntxiv ntawm endometrium.Ntawm qhov tsis sib xws, cov txiaj ntsig tsis zoo qhia tau hais tias qhov kev pheej hmoo ntawm endometrial precancerous lesions thiab mob qog noj ntshav yog tsawg, tab sis qhov kev pheej hmoo tsis tuaj yeem suav nrog tag nrho.Kev kuaj mob zaum kawg yuav tsum yog raws li kev soj ntsuam histopathological ntawm endometrium.
PCDHGB7 yog ib tug tswv cuab ntawm tsev neeg protocadherin γ gene pawg.Protocadherin tau pom los tswj cov txheej txheem lom neeg xws li cell proliferation, cell cycle, apoptosis, ntxeem tau, tsiv teb tsaws thiab autophagy ntawm cov qog hlwb los ntawm ntau txoj kev taw qhia, thiab nws cov noob ntsiag to tshwm sim los ntawm hypermethylation ntawm thaj chaw txhawb nqa yog ze ze rau qhov tshwm sim thiab kev loj hlob. ntawm ntau yam mob qog noj ntshav.Nws tau raug tshaj tawm tias hypermethylation ntawm PCDHGB7 yog txuam nrog ntau hom qog nqaij hlav, xws li non-Hodgkin lymphoma, mob qog noj ntshav mis, mob qog noj ntshav, mob qog noj ntshav, thiab qog nqaij hlav qog.
DETECTION PRINCIPLE
Cov khoom siv no muaj cov nucleic acid extraction reagent thiab PCR nrhiav reagent.Nucleic acid yog muab rho tawm los ntawm magnetic-bead-based method.Cov khoom siv no yog ua raws li lub hauv paus ntsiab lus ntawm fluorescence quantitative PCR txoj kev, siv cov tshuaj methylation-specific real-time PCR cov tshuaj tiv thaiv los soj ntsuam cov qauv DNA, thiab ib txhij tshawb xyuas CpG qhov chaw ntawm PCDHGB7 noob thiab cov cim kev tswj kom zoo hauv kev siv cov noob tawg G1 thiab G2.Cov qib methylation ntawm PCDHGB7 hauv cov qauv, lossis Me tus nqi, yog xam raws li PCDHGB7 gene methylated DNA amplification Ct tus nqi thiab Ct tus nqi ntawm kev siv.PCDHGB7 gene hypermethylation zoo lossis tsis zoo yog txiav txim siab raws li Me tus nqi.
