TAGMe DNA Methylation Detection Kits (qPCR) rau Cervical Cancer
KHOOM NTA
Precision
Validated tshaj 36000 cov qauv kuaj hauv ob qhov muag tsis pom kev ntau qhov chaw, cov khoom muaj qhov tshwj xeeb ntawm 94.3% thiab rhiab heev ntawm 96.0%.
Yooj yim
Thawj Me-qPCR methylation nrhiav tshuab tuaj yeem ua tiav hauv ib kauj ruam hauv 3 teev yam tsis muaj kev hloov pauv bisulfite.
Thaum ntxov
Kev kuaj mob khees xaws ncauj tsev menyuam tuaj yeem ua tau mus rau theem ntawm cov kab mob siab (precancerous lesions) theem.
Automation
Nrog rau cov txiaj ntsig kev tshuaj ntsuam xyuas software, kev txhais cov txiaj ntsig yog automated thiab nyeem tau ncaj qha.
YUAV TSUM SIV
Cov khoom siv no yog siv rau hauv vitro qualitative nrhiav pom ntawm hypermethylation ntawm cov noob PCDHGB7 nyob rau hauv cov qauv ncauj tsev menyuam.Cov txiaj ntsig zoo qhia tau tias muaj kev pheej hmoo siab ntawm qib 2 lossis qib siab dua / ntau dua ntawm ncauj tsev menyuam intraepithelial neoplasia (CIN2+, suav nrog CIN2, CIN3, adenocarcinoma hauv situ, thiab mob qog noj ntshav), uas yuav tsum tau kuaj ntxiv colposcopy thiab / lossis histopathological kuaj.Ntawm qhov tsis sib xws, cov txiaj ntsig tsis zoo qhia tau hais tias qhov kev pheej hmoo ntawm CIN2+ tsawg, tab sis qhov kev pheej hmoo tsis tuaj yeem raug cais tawm tag nrho.Kev kuaj mob zaum kawg yuav tsum yog nyob ntawm colposcopy thiab / lossis cov txiaj ntsig histopathological.PCDHGB7 yog ib tug tswv cuab ntawm tsev neeg protocadherin γ gene pawg.Protocadherin tau pom los tswj cov txheej txheem lom neeg xws li cell proliferation, cell cycle, apoptosis, ntxeem tau, tsiv teb tsaws thiab autophagy ntawm cov qog hlwb los ntawm ntau txoj kev taw qhia, thiab nws cov noob ntsiag to tshwm sim los ntawm hypermethylation ntawm thaj chaw txhawb nqa yog ze ze rau qhov tshwm sim thiab kev loj hlob. ntawm ntau yam mob qog noj ntshav.Nws tau raug tshaj tawm tias hypermethylation ntawm PCDHGB7 yog txuam nrog ntau hom qog nqaij hlav, xws li non-Hodgkin lymphoma, mob qog noj ntshav mis, mob qog noj ntshav, mob qog noj ntshav, thiab qog nqaij hlav qog.
DETECTION PRINCIPLE
Cov khoom siv no muaj cov nucleic acid extraction reagent thiab PCR nrhiav reagent.Nucleic acid yog muab rho tawm los ntawm magnetic-bead-based method.Cov khoom siv no yog ua raws li lub hauv paus ntsiab lus ntawm fluorescence quantitative PCR txoj kev, siv cov tshuaj methylation-specific real-time PCR cov tshuaj tiv thaiv los soj ntsuam cov qauv DNA, thiab ib txhij tshawb xyuas CpG qhov chaw ntawm PCDHGB7 noob thiab cov cim kev tswj kom zoo hauv kev siv cov noob tawg G1 thiab G2.Cov qib methylation ntawm PCDHGB7 hauv cov qauv, lossis Me tus nqi, yog xam raws li PCDHGB7 gene methylated DNA amplification Ct tus nqi thiab Ct tus nqi ntawm kev siv.PCDHGB7 gene hypermethylation zoo lossis tsis zoo yog txiav txim siab raws li Me tus nqi.
Daim ntawv thov scenarios
Kev tshuaj ntsuam thaum ntxov
Cov neeg noj qab haus huv
Kev soj ntsuam kev pheej hmoo mob qog noj ntshav
Cov neeg muaj kev pheej hmoo siab (zoo rau cov neeg muaj kev pheej hmoo siab tib neeg papillomavirus (hrHPV) lossis zoo rau lub ncauj tsev menyuam exfoliation cytology)
Kev soj ntsuam rov tshwm sim
Postoperative pejxeem (nrog keeb kwm ntawm qib siab ntawm lub ncauj tsev menyuam qhov chaw mos lossis mob qog noj ntshav)
Clinical tseem ceeb
Kev tshuaj xyuas ntxov rau cov neeg noj qab haus huv:Cervical cancer thiab precancerous lesions tuaj yeem kuaj xyuas kom raug
Kev ntsuas kev pheej hmoo hauv cov neeg muaj kev pheej hmoo siab:Kev faib cov kev pheej hmoo tuaj yeem ua tau nyob rau hauv cov pej xeem HPV-zoo los coj kev kuaj xyuas tom ntej
Kev saib xyuas rov qab rau cov neeg tom qab kev phais:Kev tshuaj xyuas cov neeg rov qab ua dua tuaj yeem ua tau los tiv thaiv kev qeeb hauv kev kho mob los ntawm kev rov ua dua
Qauv sau
Txoj kev coj mus kuaj: Muab cov khoom pov tseg ntawm ncauj tsev menyuam rau ntawm lub ncauj tsev menyuam, maj mam rub lub ncauj tsev menyuam txhuam thiab tig 4-5 zaug clockwise, maj mam tshem lub ncauj tsev menyuam txhuam, muab tso rau hauv lub cell preservation tov, thiab sau npe rau kev kuaj hauv qab no.
Kev khaws cia ntawm cov qauv:Cov qauv tuaj yeem khaws cia hauv chav sov txog li 14 hnub, ntawm 2-8 ℃ txog li 2 lub hlis, thiab ntawm -20 ± 5 ℃ txog li 24 lub hlis.
Cov txheej txheem tshawb pom: 3 Teev (Tsis muaj phau ntawv txheej txheem)
DNA Methylation Detection Kits (qPCR) rau Urothelial Cancer
| Daim ntawv thov kev kho mob | Clinical auxiliary diagnosis of cervical cancer |
| Kev kuaj gene | PCDHGB 7 |
| Hom qauv | Poj niam cev xeeb tub |
| Kev sim | Fluorescence quantitative PCR technology |
| Cov qauv siv tau | AB 7500 |
| Packing specification | 48 xeem/kit |
| Kev cia khoom | Cov khoom siv A yuav tsum khaws cia ntawm 2-30 ℃ Cov khoom siv B yuav tsum khaws cia ntawm -20 ± 5 ℃ Siv tau mus txog 12 lub hlis |
